Product Name :
3,3′,5,5′-Tetramethylbenzidine (CAS 54827-17-7)
Synonym :
TMB substrate; BM blue; Sure Blue TMB
Application :
3,3′,5,5′-Tetramethylbenzidine is a sensitive reagent for the detection of hemoglobin
CAS:
54827-17-7
Purity:
Molecular Weight:
240.34
Formula :
C16H20N2
Physical state:
Solid
solubility :
Soluble in ethyl acetate (5%). Insoluble in water.
Shipping Condition :
Store at 4° C
Melting point:
168-171° C (lit.)
SMILES:
CC1=CC(=CC(=C1N)C)C2=CC(=C(C(=C2)C)N)C
References:
:[Mechanisms of peroxidase oxidation of o-dianisidine, 3,3′,5,5′-tetramethylbenzidine, and o-phenylenediamine in the presence of sodium dodecyl sulfate]. | Kireko, AV., et al. 2006. Bioorg Khim. 32: 80-6. PMID: 16523724Double wavelength measurement of 3,3′,5,5′-tetramethylbenzidine (TMB) provides a three-fold enhancement of the ELISA measuring range. | Madersbacher, S. and Berger, P. 1991. J Immunol Methods. 138: 121-4. PMID: 1902244Investigation of 3,3′,5,5′-tetramethylbenzidine as colorimetric substrate for a peroxidatic DNAzyme. | Li, B., et al. 2009. Anal Chim Acta. 651: 234-40. PMID: 19782817Highly sensitive and selective colorimetric detection of glutathione based on Ag [I] ion-3,3′,5,5′-tetramethylbenzidine (TMB). | Ni, P., et al. 2015. Biosens Bioelectron. 63: 47-52. PMID: 25058938Selective extraction and release using (EDTA-Ni)-layered double hydroxide coupled with catalytic oxidation of 3,3′,5,5′-tetramethylbenzidine for sensitive detection of copper ion. | Tang, S., et al. 2015. Anal Chim Acta. 885: 106-13. PMID: 26231895Selective sensing of submicromolar iron(III) with 3,3′,5,5′-tetramethylbenzidine as a chromogenic probe. | Zhang, L. and Du, J. 2016. Spectrochim Acta A Mol Biomol Spectrosc. 158: 24-8. PMID: 26783724A simple and novel colorimetric assay for tyrosinase and inhibitor screening using 3,3′,5,5′-tetramethylbenzidine as a chromogenic probe. | Lei, C., et al. 2017. Talanta. 175: 457-462. PMID: 28842016Colorimetric Sensor Arrays for Antioxidant Discrimination Based on the Inhibition of the Oxidation Reaction between 3,3′,5,5′-Tetramethylbenzidine and Hydrogen Peroxides. | Li, X., et al. 2019. ACS Appl Mater Interfaces. 11: 9504-9509. PMID: 30735025A system composed of polyethylenimine-capped upconversion nanoparticles, copper(II), hydrogen peroxide and 3,3′,5,5′-tetramethylbenzidine for colorimetric and fluorometric determination of glyphosate. | Liu, Z., et al. 2019. Mikrochim Acta. 186: 835. PMID: 31758331Rationale of 3,3′,5,5′-Tetramethylbenzidine as the Chromogenic Substrate in Colorimetric Analysis. | Zhang, X., et al. 2020. Anal Chem.455-86-7 manufacturer 92: 12400-12406.34759-34-7 Synonym PMID: 327947053,3′,5,5′-tetramethylbenzidine as multi-colorimetric indicator of chlorine in water in line with health guideline values.PMID:33688785 | Palladino, P., et al. 2020. Anal Bioanal Chem. 412: 7861-7869. PMID: 32870352A colorimetric method for screening alpha -glucosidase inhibitors from flavonoids using 3,3′,5,5′-tetramethylbenzidine as a chromogenic probe. | Liu, DM., et al. 2021. Colloids Surf B Biointerfaces. 197: 111400. PMID: 33113490Integration and Quantitative Visualization of 3,3′,5,5′-Tetramethylbenzidine-Probed Enzyme-Linked Immunosorbent Assay-like Signals in a Photothermal Bar-Chart Microfluidic Chip for Multiplexed Immunosensing. | Fu, G., et al. 2021. Anal Chem. 93: 15105-15114. PMID: 34734693The use of 3,3′,5,5′-tetramethylbenzidine as a peroxidase substrate in microplate enzyme-linked immunosorbent assay. | Goka, AK. and Farthing, MJ. 1987. J Immunoassay. 8: 29-41. PMID: 35588273,3′,5,5′ – Tetramethylbenzidine as an Ames test negative chromogen for horse-radish peroxidase in enzyme-immunoassay. | Bos, ES., et al. 1981. J Immunoassay. 2: 187-204. PMID: 7047570