HCl (pH 7.4), 200 mM NaCl, 1 mM dithiothreitol (DTT), 100 M ZnSO4, and 10 mM
HCl (pH 7.4), 200 mM NaCl, 1 mM dithiothreitol (DTT), 100 M ZnSO4, and ten mM maltose. In vitro ubiquitylation assays had been performed primarily as described previously having a…