PMI-1640 media; Chang Liver, HEK-293, Hep G2, MDA-MB-231, NRK-52E, RAW 264.7, Vero, and WRL 68 had been maintained in DMEM; whilst Caco-2, HK1, and HSC-2 have been grown in MEM. All media had been supplemented with ten (v/v) heat-inactivated fetal bovine serum (FBS) and 100 units/ml of penicillin/streptomycin. The NP 69 and OKF6 lines have been cultured in keratinocyte serum-free media (Keratinocyte-SFM, Invitrogen) supplemented with L-glutamine, human epidermal development issue (hEGF, 0.1 ng/ml), bovine pituitary extract (BPE, 50 mg/ml), and Ca2+ (final concentration,Table 1. Antioxidant capacity of L. rhinocerotis extracts.Extracts LR-MH LR-MT LR-BH LR-BT LR-SC*DPPH (IC50, mg/ml) 0.9460.01 a three.7260.11 b four.2360.08 c 6.8760.06 d 3.6060.ten bTEAC (mmol trolox equiv./g extract) 143613.42 a 128.4369.25 a 186.6767.Formula of 3-(Difluoromethyl)aniline 54 a, b 223.0568.26 b 162.93624.63 a, bFRAP (mmol FeSO4?7H2O equiv./g extract) 71.2561.91 a 21.2161.04 b 67.0263.00 a 85.7364.02 c 23.0161.31 bMetal chelating CUPRAC (mmol Na2EDTA (mmol Trolox equiv./g equiv./g extract) extract) 350.4165.15 a 214.33611.66 b, c 274.7867.34 d 268.0165.61 a, d 192.53615.86 b 40.4460.07 a 31.9761.68 b 26.7660.50 c 59.4360.36 d 57.9560.14 dInhibition of lipid peroxidation (mmol MDA/g extract) 1.5160.08 a 1.5560.05 a 1.8960.09 a 1.4860.04 b 1.5960.02 aThe extracts had been dissolved in 50 (v/v) methanol in water for the antioxidant assays. Benefits were expressed as imply six SE of at the least three independent experiments (n = 3?) performed in triplicates. The various letters (a ) within a column represent means with significance distinction (p,0.Potassium osmate dihydrate Purity 05). *Quercetin dihydrate (IC50: 0.091 mg/ml) was employed as the positive handle within the DPPH free of charge radical scavenging assay. doi:ten.1371/journal.pone.0102509.tPLOS 1 | plosone.orgTable two. Cytotoxic effect of L. rhinocerotis extracts.Cell lines and extracts LR-MH 20 98.162.37 99.262.42 90.460.89 110.064.09 99.963.32 104.160.63 91.265.70 101.061.63 111.462.75 98.263.71 91.665.72 110.363.64 109.160.19 81.260.87 107.965.02 93.760.76 91.263.29 99.964.66 101.361.85 104.363.68 98.964.35 92.764.07 89.760.76 69.961.16 105.863.32 91.862.89 83.760.68 94.062.24 85.861.00 84.661.66 88.863.82 97.462.00 75.460.58 103.961.03 118.761.43 98.560.11 84.263.35 89.462.93 92.060.64 93.061.09 92.862.54 68.360.16 89.061.11 65.662.83 105.662.19 84.262.82 102.461.29 75.562.21 113.961.60 65.460.20 79.660.74 62.162.29 79.660.68 67.561.97 97.661.23 103.161.69 86.565.43 107.864.26 117.262.55 102.760.42 68.862.23 89.861.80 109.763.44 77.560.17 102.660.71 85.063.24 81.461.95 102.162.57 37.164.34 100.666.95 107.363.23 92.161.01 92.764.88 85.763.03 83.362.95 77.562.51 90.460.94 101.362.09 94.561.53 90.960.88 91.161.27 110.867.96 106.261.64 71.663.PMID:33574180 87 114.162.17 108.763.09 124.065.33 95.061.25 104.760.08 102.366.54 102.262.40 79.160.82 112.963.40 105.760.48 94.660.91 102.363.73 123.364.21 97.860.19 87.665.44 83.360.84 108.161.97 96.461.87 119.663.86 one hundred.962.11 124.263.03 89.465.08 92.061.10 93.061.88 92.864.40 86.569.41 94.661.58 one hundred.161.16 99.860.44 88.662.22 102.162.53 90.062.54 one hundred.162.05 85.563.25 102.361.69 76.661.21 93.863.79 70.762.20 94.266.77 69.865.49 103.961.17 93.165.29 101.161.31 113.161.61 97.762.11 95.663.50 83.264.69 82.161.41 77.560.69 113.961.33 105.460.57 93.163.05 94.763.22 132.760.64 104.761.21 86.966.07 92.261.68 93.760.64 81.063.38 111.265.9 107.761.24 111.660.33 104.360.14 99.061.78 101.161.23 93.061.13 103.460.77 99.560.97 102.562.07 98.061.35 91.663.02 97.162.07 79.362.89 95.060.94 79.963.25 98.465.46 8.
