Product Name :
Phospho-Ezrin (T567)/Radixin (T564)/Moesin (T558) Recombinant Rabbit Monoclonal Antibody [PSH05-73]
Predicted band size :
69 kDa
Observed band size :
75/80 kDa
Synonyms:
Villin 2 ezrin antibody CVIL antibody CVL antibody Cytovillin 2 antibody Cytovillin antibody DKFZp762H157 antibody Epididymis secretory protein Li 105 antibody EZR antibody EZRI_HUMAN antibody Ezrin antibody 展开
Function :
The ezrin, radixin, and moesin (ERM) proteins function as linkers between the plasma membrane and the actin cytoskeleton and are involved in cell adhesion, membrane ruffling, and microvilli formation . ERM proteins undergo intra or intermolecular interaction between their amino- and carboxy-terminal domains, existing as inactive cytosolic monomers or dimers. Phosphorylation at a carboxy-terminal threonine residue (Thr567 of ezrin, Thr564 of radixin, Thr558 of moesin) disrupts the amino- and carboxy-terminal association and may play a key role in regulating ERM protein conformation and function.529476-80-0 web Phosphorylation at Thr567 of ezrin is required for cytoskeletal rearrangements and oncogene-induced transformation. Ezrin is also phosphorylated at tyrosine residues upon growth factor stimulation. Phosphorylation of Tyr353 of ezrin transmits a survival signal during epithelial differentiation.
Antibody Type:
Recombinant Rabbit monoclonal Antibody
Immunogen :
Synthetic phosphopeptide corresponding to residues surrounding Thr567 of human ezrin protein.
Species reactivity:
Human, Mouse, Rat
Verify application:
WB, IF-Cell, IHC-P
Coupling :
unconjugated
Clone number:
PSH05-73
RRID:
Form :
Liquid
Concentration :
1ug/ul
Storage instructions:
Store at +4℃ after thawing.1227598-69-7 web Aliquot store at -20℃.PMID:33530718 Avoid repeated freeze / thaw cycles.
Storage buffer solution:
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Subtype:
IgG
Purification method :
Protein A affinity purified.
ELTSA :
Molecular weight :
Predicted band size: 69 kDa
Positive control :
HeLa starved for 3 hours then add 100nM Calyculin A for 30 minutes cell lysate, NIH/3T3 starved for 24 hours then add 100nM Calyculin A for 30 minutes cell lysate, C6 treated with 100ng/mL Calyculin A for 1 hours cell lysate, mouse liver tissue, rat liver tissue.